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    动物源性鉴定

    项目背景:
            目前市场上肉(制)品种掺杂、掺假的现象时有报道,部分不法商贩为了谋求不正当利益,在肉(制)品中掺加其他来源的品种,甚至全部为标称以外的其他价格便宜的动物源性成分,例如牛肉(制品)中掺杂猪源性成分、羊肉制品中掺杂鸭成分、马肉冒充驴肉等。

            动物源性饲料中的牛羊源性成分被认为是牛海绵状脑病、痒病传播的主要原因,禁止疫区含牛羊源性成分的动物源性饲料的生产、流通和使用,已成为防止动物疾病感染、流行的主要手段。

            通过传统感官鉴别方法如其色泽、气味、组织性状等对于肉及肉制品的鉴别检测,已不能满足食品安全管理的需要,尤其是对一些添加了色素、芳香剂、深加工的肉制品,感官判断可靠性更差,而饲料中的动物源性成分用常规方法就更无法分辨。

            荧光PCR技术利用发光技术对PCR扩增过程进行实时检测,能够准确对样品中DNA的初始量进行定量,具有特异性好、灵敏度高等特点,可对肉及肉制品中动物源性成分进行快速、准确鉴别。

    检测标准:
    SN/T 2051-2008食品、化妆品和饲料中牛羊猪源性成分检测方法实时PCR法
    SN/T 2980-2011动物产品中牛、山羊和绵羊源性成分三重实时荧光PCR检测方法
    SN/T 3730.1-2013食品及饲料中常见畜类品种的鉴定方法第1部分:貂成分检测实时荧光PCR法
    SN/T 3730.2-2013食品及饲料中常见畜类品种的鉴定方法第2部分:狗成分检测实时荧光PCR法
    SN/T 3730.3-2013食品及饲料中常见畜类品种的鉴定方法第3部分:狐狸成分检测实时荧光PCR法
    SN/T 3730.4-2013食品及饲料中常见畜类品种的鉴定方法第4部分:驴成分检测实时荧光PCR法
    SN/T 3730.5-2013食品及饲料中常见畜类品种的鉴定方法第5部分:马成分检测实时荧光PCR法
    SN/T 3730.6-2013食品及饲料中常见畜类品种的鉴定方法第6部分:猫成分检测实时荧光PCR法
    SN/T 3730.7-2013食品及饲料中常见畜类品种的鉴定方法第7部分:水牛成分检测实时荧光PCR法
    SN/T 3730.8-2013食品及饲料中常见畜类品种的鉴定方法第8部分:猪成分检测实时荧光PCR法
    SN/T 3731.5-2013食品及饲料中常见禽类品种的鉴定方法第5部分鸭成分检测PCR法
    SN/T 2978-2011动物源性产品中鸡源性成分PCR检测方法

    检测项目:
    食品中动物源性成分检测
    牛、羊、猪、貂、狗、狐狸、驴、马、猫、水牛、鸭、鸡、鲨鱼等。
    饲料中动物源性成分检测
    牛、羊、猪、貂、狗、狐狸、驴、马、猫、水牛、鸭、鸡、骆驼、鹿、兔、反刍动物、哺乳动物等。

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